Hu-binding site
Web1 Mar 2008 · HU binding to ssDNA, dsDNA and nick in low salt conditions. HU protein, at the concentration indicated in nM on the bottom, was mixed with end-labeled DNA, 40-bp long dsDNA, the same DNA with a ... WebBinding Site provides specialist diagnostic products to clinicians and laboratory professionals worldwide. Our people are dedicated to improving patient lives, …
Hu-binding site
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Web4 Feb 2009 · In these conditions, the average spacing between HU binding sites on the chromosome would amount to ∼20 kb (Supplemental Figure S2). Interestingly, the bacterial nucleoid has been described as being shaped in domains of 50–100 kb [80] whereas more recent studies re-evaluated the organization of the chromosome in 400 supercoiled … Web30 Aug 2024 · The identification of metal ion binding sites is important for protein function annotation and the design of new drug molecules. This study presents an effective …
Web8 Jan 2024 · Transcription factor binding sites (TFBSs) are basic components of cis-regulatory elements, which act important roles in precise regulations of gene expressions … Initially, bacterial DNA binding proteins were thought to help stabilize bacterial DNA. Currently, many more functions of bacteria DNA binding proteins have been discovered, including the regulation of gene expression by histone-like nucleoid-structuring protein, H-NS. H-NS is about 15.6 kDa and assists in the regulation of bacterial transcription i…
Web19 Jun 2008 · The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA. Web1 Dec 2000 · Introduction. Escherichia coli HU, a small, basic, heat-stable DNA binding protein, is one of the most abundant proteins associated with the E.coli nucleoid (Rouviere-Yaniv and Gros, 1975; Rouviere-Yaniv, 1978). In vitro, HU shares the ability observed with histones to introduce negative supercoiling into relaxed DNA molecules in the presence …
WebHU, a major component of the bacterial nucleoid, shares properties with histones, high mobility group proteins (HMGs), and other eukaryotic proteins. HU, which participates in many major pathways of the bacterial cell, binds without sequence specificity to duplex DNA but recognizes with high affinity DNA repair intermediates. international youth for christ of the wotccWeb7 Apr 2024 · ELAV/Hu RNA binding proteins determine multiple programs of neural alternative splicing - PMC Back to Top Skip to main content An official website of the United States government Here's how you know The .gov means it’s official. Federal government websites often end in .gov or .mil. international youth football tournaments 2017Web1 Nov 1996 · The structure of the NSAID binding site is also well conserved, although there are differences in its overall size and shape which may be exploited for the further … international youth hostel federation iyhfWebbound to its recognition site. Binding of HU is sequence un-speci¢c but, as is the case with IHF [7], binding to DNA probably involves its two arms being inserted into the minor groove. H-NS is similar in size to HU (15.6 kDa) and also binds DNA without sequence speci¢city. Unlike HU, this protein newcastle university gpWebPapain-like protease (PLpro) is a promising therapeutic target against SARS-CoV-2, but its restricted S1/S2 subsites pose an obstacle in developing active site-directed inhibitors. We have recently identified C270 as a novel covalent allosteric site for SARS-CoV-2 PLpro inhibitors. Here we present a theoreti newcastle university gender equality planWeb22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray … newcastle university gown hireWeb29 Apr 2014 · Site-directed mutagenesis of the putative C/EBPα binding sites decreased FTO promoter activity. Overexpression and RNAi studies also indicated that C/EBP α was required for the expression of FTO. Chromatin immunoprecipitation (ChIP) experiment was carried out and the result shows direct binding of C/EBP α to the putative binding … newcastle university gps staff