WebMar 21, 2024 · The PrpHluorin-AtVSR2 (PVC/MVB-targeted pH sensor) was transiently expressed in the rice protoplasts (Shen et al., 2013, Yoo et al., 2007, Zhu et al., 2024). … WebFeb 18, 2024 · Whereas the role of calcium ions (Ca 2+ ) in plant signaling is well studied, the physiological significance of pH-changes remains largely undefined. Here we developed CapHensor,

pH-sensitive Fluorescent Proteins and Their Applications in Plant …

WebEl Prof. Antonio Di Pietro y el Dr. David Turrá, director y tutor, respectivamente, del trabajo titulado “In vivo determination of intracellular pH using pHluorin proteins in Fusarium … WebDec 5, 2013 · A plant dual-excitation ratiometric pHluorin, PRpHluorin—which is similar to PEpHluorin —is a fusion between a soluble modified GFP (smGFP) with F99S mutation and pHluorin with additional mutations M153T and V163A . The presence of smGFP decreases pK a to 6.6 with no effect on the spectral waveforms of pHluorin. Hanson et al. swiss peak

An optimized genetically encoded dual reporter for …

WebIn situ calibration of PEpHluorin and PRpHluorin . (a) A plot of the fluorescence intensity versus intracellular pH between 5.5 and 8.5.The fluorescence intensity after excitation λ … Websupplementary files: Fig. S1 Isosbestic point of PRpHluorin in pollen tubes and guard cells. Fig. S2 Ca 2+ binding to YC3.6 and R-GECO1 in vitro.. Fig. S3 [Ca 2+] cyt and [H +] cyt … WebThe PRpHluorin-HDEL signals at emission wavelength of 500 to 530 nm were recorded with dual-excitation wavelength at 405 and 488 nm, respectively, and obtained a ratio … brava crema porcelain paving